一、基本信息
姓名：刘元建
职称/职务：学科教授、食品系主任
联系电话/电子邮箱：lucias_cumt@163.com
二、教育背景（包括学校、专业、时间）：
2012/09-2017/06，东南大学，化学化工学院，博士，导师：卫伟/刘松琴
三、研究和工作经历：
2017/11-2019/06，南京工业大学，食品与轻工学院，讲师
2019/07-2025.12，南京工业大学，食品与轻工学院，副教授
2026/01-至今，南京工业大学，食品与轻工学院，学科教授
四、境外经历（如有，请注明出访国家、时间、主要内容）：
/
五、学术兼职（包括机构、职务、起止时间）：
江苏省产品质量鉴定专家
江苏省食品科学与技术学会理事
江苏省食品生产许可注册审查员
六、研究方向：
主要从事食源性致病菌和食品中典型化学污染物快速检测方法与设备开发。
七、教育教学方面（主讲课程、教学改革、指导学生等）：
主讲《实验设计与数据处理》、《仪器分析专题》本科生课程。2025年指导22级本科生获第十届全国大学生生命科学竞赛一等奖。
八、科研工作方面：
在主流学术期刊发表论文50篇，引用1060余次，H因子14。第一/通讯作者论文36篇，如Food Chemistry、Analytical Chemistry、Chemical Communications和Biosensors & Bioelectronics等。多次担任Sensors and Actuators: B. Chemical、Analytica Chimica Aata、Analyst等国际著名学术期刊特邀审稿人。授权中国发明专利7项，承担国家自然科学基金青年基金项目1项，江苏省自然科学基金青年基金项目1项，企事业单位检验检测项目17项。
针对现有食源性致病菌检测面临灵敏度低、特异性差、抗基质干扰能力弱等问题，开发了一系列基于双极电极的电化学生物传感器，结合电致化学发光、比色等信号输出方式，实现了对单增李斯特菌、大肠杆菌O157:H7、沙门氏菌等常见食源性致病菌的高灵敏、高特异性可视化检测，相关技术已经形成了稳定的知识产权布局，可为现场快速检测提供核心技术支撑。
针对现有食品快检技术及快检产品前处理手段缺乏、产品特别是基于酶抑制法农残检测产品的假阳性假阴性率较高、检测目标单一等缺陷，以食品非法添加物、农兽药残留、真菌毒素和类激素等典型化学污染物为研究对象，设计、构筑一系列对食品典型化学污染物具有高效富集和选择性分离作用的功能化纳米材料、分子印迹膜和抗体/适体，阐明其与残留危害物质的相互识别、构效关系及其吸附解析机制，研究开发对化学污染物具备特异性识别、高效富集、净化的样品前处技术及材料，研发高效全自动全过程智能化前处理设备；利用量子点和其他多色荧光纳米探针标记技术，结合微纳加工技术，发展食品复杂基质中农兽药残留、真菌毒素、类激素、非食品添加物等典型化学污染物高通量、多目标、高灵敏的快速检测方法。
九、代表性成果：
一、发表论文：
1、Fengyang Wang, Panpan Liu, Yin Chen, Mengjuan Li, Yunlong Liu, Yan Qi, Xiaohui Xiong, Yuanjian Liu*. Electrochemical detection of E. coli O157:H7 based on NaCl stress improving the bacterial electrocatalytic oxygen reduction performance. Food Chemistry, 2026, 516, 149305.
2、Yin Chen, Panpan Liu, Fengyang Wang, Yan Qi, Xiaohui Xiong, Yuanjian Liu*. Construction of a shared-cathode closed bipolar electrode for type 2 diabetes mellitus preliminary screening. Analytical Methods, 2025, 17, 9557-9564.
3、Fengyang Wang, Panpan Liu, Mengjuan Li, Yunlong Liu, Yan Qi, Xiaohui Xiong, Yuanjian Liu*. Electrocatalytic oxygen reduction activity of E. coli O157:H7 under H2O2 stress for biosensing application. ACS Sensors, 2025, 10, 7842−7851.
4、Yunlong Liu, Panpan Liu, Mengjuan Li, Fengyang Wang, Yusong Wan, Yan Qi, Lei Ji, Xiaohui Xiong, Yuanjian Liu*. Detection of E. coli O157:H7 using a molecularly imprinted bacterial bipolar electrode and an Au@metal-organic framework. Analytical Methods, 2025, 17, 8005-8013.
5、Yuanjian Liu*, Yan Qi, Panpan Liu, Yusong Wan, Fengyang Wang, Yunlong Liu, Xiaohui Xiong. Highly sensitive detection of Escherichia coli O157:H7 via β-galactosidase-PNPG/pNP signal amplification assisted bipolar electrode-electrochemiluminescence. Microchemical Journal, 2025, 215, 114555.
6、Yan Qi, Fengyang Wang, Yanjun Jiang, Lei Ji, Xiaohui Xiong, Yuanjian Liu*. A bipolar electrode-electrochemiluminescence sensor for sensitive detection of escherichia coli O157:H7 based on loop-mediated isothermal amplification. Food Analytical Methods, 2025, 18(6), 1180-1188.
7、Mengjuan Li, Yan Qi, Mingyao Xu, Fengyang Wang, Xiaohui Xiong, Yuanjian Liu*. Rapid screening of Salmonella typhimurium based on a bacterial surface-imprinted polymer-functionalized bipolar electrode-electrochemiluminescence platform. Microchemical Journal, 2024, 207, 111788.
8、Lei Ji, Fengyang Wang, Yan Qi, Fanglin Qiao, Xiaohui Xiong, Yuanjian Liu*. Detection of pathogenic gram-negative bacteria using an antimicrobial peptides-modified bipolar electrode-electrochemiluminescence platform. Microchimica Acta, 2024, 191, 648.
9、Fengyang Wang, Yan Qi, Lei Ji, Fanglin Qiao, Yin Chen, Xiaohui Xiong, Yuanjian Liu*. Visual detection of ochratoxin a based on GPE-PET bipolar electrode-electrochemiluminescence platform. Food Chemistry, 2024, 461, 140842.
10、Fanglin Qiao, Yin Chen, Yunjie Fei, Yanjun Jiang, Xiaohui Xiong, Yuanjian Liu*. An antibody ordered assembly functional BPE-ECL platform for aflatoxin B1 detection. Food Analytical Methods, 2024, 17(2), 260-268.
11、Lijun Lin, Yanjun Jiang, Yin Chen, Lei Ji, Mingyao Xu, Yuanjian Liu*, Xiong Xiaohui. A double bipolar electrode electrochemiluminescence device for rapid visual detection of organophosphorus pesticides. Microchemical Journal, 2023, 195, 109191.
12、Mingyao Xu, Yin Chen, Yunjie Fei, Fenglan Lv, Xiaohui Xiong, Yuanjian Liu*. A bipolar electrode-electrochemiluminescence sensor for Salmonella typhimurium detection based on β-cyclodextrin host-guest recognition. Microchemical Journal, 2023, 193, 109507.
13、Yunjie Fei#, Ning Tang#, Yin Chen, Mingyao Xu, Qin Tao, Yuanjian Liu*, Xiaohui Xiong. Rapid screening of electrochemically active bacteria based on a biocathode-functional bipolar electrode-electrochemiluminescence platform. Food Chemistry, 2023, 429, 136919.
15、Qin Tao#, Ning Tang#, Yanjun Jiang, Bin Chen, Yuanjian Liu*, Xiaohui Xiong, Songqin Liu. Double bipolar electrode electrochemiluminescence color switch for food-borne pathogens detection. Biosensors and Bioelectronics, 2023, 237, 115452.
15、Qin Tao#, Ning Tang#, Songtao OuYang, Yanjun Jiang, Yun Luo, Yuanjian Liu*, Xiaohui Xiong. Rapid visual screening of OTA based on multicolor electrochemiluminescence. Food Analytical Methods, 2023, 16(3), 491-498.
16、Yunjie Fei , Yuanjian Liu*, Xiaohui Xiong. Development and present situation of detection techniques for foodborne pathogens[J]. Chinese Journal of Bioprocess Engineering, 2023, 21(3): 308-325.
17、刘元建，林丽军，姚丽丽，崔晓文. 新时代食品质量与安全专业人才培养模式探索. 科教导刊（电子版）, 2024年第13期（5月）, 16-18.
18、Bin Chen#, Qin Tao#, Songtao OuYang, Minhui Wang, Yuanjian Liu*, Xiaohui Xiong, Songqin Liu*. Biocathodes reducing oxygen in BPE-ECL system for rapid screening of E. coli O157:H7. Biosensors and Bioelectronics, 2023, 221, 114940.
19、Bin Chen#, Qin Tao#, Fanglin Qiao, Yunjie Fei, Yuanjian Liu*, Xiaohui Xiong, Songqin Liu*. Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection. Food Chemistry, 2022, 383, 132404.
20、Yun Luo#, Fenglan Lv#, Minhui Wang, Lixia Lu, Yuanjian Liu*, Xiaohui Xiong. A multicolor electrochemiluminescence device based on closed bipolar electrode for rapid visual screening of Salmonella typhimurium. Sensors and Actuators: B. Chemical, 2021, 349, 130761.
21、Wei Yuan#, Lixia Lu#, Yichen Lu, Xiong Xiong, Yi Li, Xiaowen Cui, Yuanjian Liu*, Xiaohui Xiong. Synergistic effects of DNA structure for ultrasensitive detecting OTA in grains. Food Analytical Methods, 2021, 14(11), 2308-2316.
22、Lixia Lu#, Wei Yuan#, Qiang Xiong, Minhui Wang, Yuanjian Liu*, Min Cao*, Xiaohui Xiong. One-step grain pretreatment for ochratoxin A detection based on bipolar electrode-electrochemiluminescence biosensor. Analytica Chimica Acta, 2021, 1141, 83-90.
23、Xiaohui Xiong, Wei Yuan, Yafei Li, Yichen Lu, Xiong Xiong, Yi Li, Yuanjian Liu*, Lixia Lu*. Sensitive electrochemical detection of aflatoxin B1 using DNA tetrahedron-nanostructure as substrate of antibody ordered assembly and template of aniline polymerization. Food Chemistry, 2020, 331, 127368.
24、Hongyu Zhao#, Minhui Wang#, Xiaohui Xiong, Yuanjian Liu*, Xiaoye Chen*. Simultaneous fluorescent detection of multiplexed miRNA of liver cancer based on DNA tetrahedron nanotags. Talanta, 2020, 210, 120677.
25、Xiaohui Xiong, Yafei Li, Wei Yuan, Yichen Lu, Xiong Xiong, Yi Li, Xiaoye Chen*, Yuanjian Liu*. Screen printed bipolar electrode for sensitive electrochemiluminescence detection of aflatoxin B1 in agricultural products. Biosensors and Bioelectronics, 2020, 150, 111873.
26、Xiaohui Xiong, Yun Luo, Yichen Lu, Xiong Xiong, Yi Li, Yuanjian Liu*, Lixia Lu*. Ultrasensitive detection of Staphylococcal enterotoxin B in milk based on target-triggered assembly of the flower like nucleic acid nanostructure. RSC Advances, 2019, 9, 42423-42429.
27、Xiaoye Chen, Yun Liu, Yichen Lu, Xiong Xiong, Yi Li, Yuanjian Liu*, Xiaohui Xiong. Chronocoulometric aptamer based assay for staphylococcal enterotoxin B by target-triggered assembly of nanostructured dendritic nucleic acids on a gold electrode. Microchimica Acta, 2019, 186: 109.
28、Xiaoye Chen, Xinping Shi, Yun Liu, Lixia Lu, Yichen Lu, Xiong Xiong, Yuanjian Liu*, Xiaohui Xiong. Impedimetric determination of Staphylococcal enterotoxin B using electrochemical switching with DNA triangular pyramid frustum nanostructure. Microchimica Acta, 2018, 185: 10.
29、Xiaohui Xiong, Xinping Shi, Yuanjian Liu*, Lixia Lu, Jingjing You. An aptamer-based electrochemical biosensor for simple and sensitive detection of staphylococcal enterotoxin B in milk. Analytical Methods, 2018, 10(3), 365-370.
30、Yuanjian Liu, Min Wei, Ying Li, Anran Liu, Wei Wei*, Yuanjian Zhang, Songqin Liu. Application of spectral crosstalk correction for improving multiplexed microRNA detection using a single excitation wavelength. Analytical Chemistry, 2017, 89(6), 3430-3436.
31、Jiahui Fan#, Yuanjian Liu#, Ensheng Xu, Yuanjian Zhang, Wei Wei*, Lihong Yin, Yuepu Pu, Songqin Liu. A label-free ultrasensitive assay of 8-hydroxy-2'-deoxyguanosine in human serum and urine samples via polyaniline deposition and tetrahedral DNA nanostructure. Analytica Chimica Acta, 2016, 943, 48-55.
32、Linqun Zhang#, Yuanjian Liu#, Ying Li, Yuewu Zhao, Wei Wei, Songqin Liu*. Sensitive electrochemical assaying of DNA methyltransferase activity based on mimic-hybridization chain reaction amplified strategy. Analytica Chimica Acta, 2016, 933(24), 75-81.
33、Yuanjian Liu, Min Wei, Linqun Zhang, Yuanjian Zhang, Wei Wei*, Lihong Yin, Yuepu Pu, Songqin Liu. Chiroplasmonic assemblies of gold nanoparticles for ultrasensitive detection of 8-hydroxy-2'-deoxyguanosine in human serum sample. Analytical Chemistry, 2016, 88(12), 6509-6514.
34、Yuanjian Liu, Min Wei, Xu Liu, Wei Wei*, Hongyu Zhao, Yuanjian Zhang, Songqin Liu. Label-free ultrasensitive detection of telomerase activity via multiple telomeric hemin/G-quadruplex triggered polyaniline deposition and a DNA tetrahedron-structure regulated signal. Chemical Communications, 2016, 52(9), 1796-1799.
35、Yuanjian Liu, Min Wei, Linqun Zhang, Wei Wei*, Yuanjian Zhang, Songqin Liu. Evaluation of DNA methyltransferase activity and inhibition via chiroplasmonic assemblies of gold nanoparticles. Chemical Communications, 2015, 51(76), 14350-14353.
36、Yuanjian Liu#, Linqun Zhang#, Wei Wei*, Hongyu Zhao, Zhenxian Zhou, Yuanjian Zhang, Songqin Liu. Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles. Analyst, 2015, 140(12), 3989-3995.
二、取得自主知识产权情况：
1. 刘元建，陶钦，熊晓辉，一种双信号适配体传感器及其制备方法和应用，专利号：ZL202211098874.6
2. 刘元建，费韵洁，熊晓辉，一种基于壳聚糖衍生物检测液体样品中食源性致病菌的方法，专利号：ZL202211693683.4
3. 刘元建，王敏慧，熊晓辉，一种可视化BPE-ECL技术检测大肠杆菌的方法，专利号：ZL202110173362.0
4. 刘元建，李亚飞，熊晓辉，基于可视化BPE-ECL技术检测黄曲霉毒素B1的方法，专利号：ZL201910874401.2
5. 刘元建，袁伟，熊晓辉，基于HRP催化聚苯胺原位生成用于AFB1的检测方法，专利号：ZL201910867875.4
6. 卫伟，刘元建，刘松琴，一种金纳米粒子探针比色法检测甲型流感病毒H3N2的方法，专利号：ZL201510152842.3
7. 卫伟，刘元建，刘松琴，一种通过荧光法同时检测多种miRNA的方法，专利号：ZL201710011874.0
十、荣誉、奖励及其他：
刘元建, 博士研究生国家奖学金, 中华人民共和国教育部, 2016
